S14 - Session P5 - Development of pathosystems to test the efficiency of news alternatives methods of treatments

Authors: Geoffrey Orgeur *, Valérie Grimault

To achieve one of the objectives of the Ecophyto plan, which is to reduce the use of phytosanitary products, many professionals in the seed sector are currently developing new alternative methods of treatment. To be able to evaluate the efficiency of these treatments, tools have been developed in the phyto pathology laboratory of GEVES in order to meet the needs of the solutions providers. To reach this goal, the capacity of transmission of the pathogen from seed to plant ( pathosystem ) is being evaluated and the effectiveness of these alternative treatment methods is being assessed. Protocols have been set up t o obtain soil or seeds infested by different pests, evaluate the percentage of infection and germination of these seeds. Depending o n the pest and treatment used, viability and damage potential of the pathogens can be evaluated using the existing detection methods (grow out, culture on media…). New biotests and experimental design have been developed and experimental approach will be presented as well as new complementary methods (vital staining, bio test ...). With the development of the alternative way of treatment, more than ever before, the presence of pathogens on seeds needs to be detected and their damage potential needs to be assessed.