S14 - Session P5 - Validation of a Ascochyta rabiei detection method on Chickpea seeds

Authors: Isabelle Serandat *, Lorine LE DARÉ, Céline LE GUISQUET, Agathe PENANT, Quentin LAMBERT, Valérie Grimault

Blight of chickpea caused by Ascochyta rabiei is a not well-known disease with a s ign i ficant impact on yield s in both organic and conventional farming. The AsCoLup project, funded under the CASDAR IP 2019, bring ing together 15 partners aims to provide producers with knowledge on Ascochyta rabiei as well as an adapted technical itinerary in order to improve disease management, both in seed s and food production. Pathogen detection is an important part of the technical itinerary . It contributes to the guarantee of production of healthy seed s and to the evaluation of the risk in the field . The international validation of a seed detection method is essential for disease management. A quantitative method on media has been chosen and the performance criteria (analytical sensitivity, analytical specificity, accuracy, repeatability and reproducibility) are studied to validate the method . A pathogenicity test is also developed to verify the pathogenicity of isolates. The validation of this method will lead to a proposal of an ISTA method on a new pathogen/host combination.