S20 - Session O1 - Development of a micro-NIR and SAW nanobiosensor to measure polyphenols in must/wine on-time and on-line

Authors: Fabio Mencarelli *, Andrea Bellincontro, raffaele cerreta, angela zinnai, isabella taglieri, chiara sanmartin, marco cecchini, matteo agostini, mariacristina gagliardi

The need for measuring polyphenols during the vinification process is an old story. Today the most up-to-date non-destructive instrument used in wineries is the FOSS, Winescan, based on FT-IR technology. But wineries need something which measures polyphenols during the extraction in order to automatize the process. We have followed two lines of research: one is to develop a low cost micro-NIR instrument and the other the development of a biosensor based on protein binding to polyphenol. In the first case, three types of instruments have been used: a homemade visible-Herschel on arduino platform, a micro FT-NIR, and a homemade visible micro sensor on arduino platform. Spectra have been aquired on different grape must varieties, wines and chemomestrics, to elaborate the correlation of spectra with destructive analyses, revealed an acceptable level of confidence but still not completely reliable. Now we are testing a new NIR technology covering from 1000 through 2500 nm which should provide the final prototype. The other research is based on the application of SAW (surface acoustic waves) with selected protein to bind polyphenols. The SAW resonator biosensor (BSA or gelatin) placed on a quarz microbalance on a chip was able to measure accurately different kinds of tannins. Further proteins are being tested.