S04 - Session P6 - Comparison of in vitro multiplication rates of Cordyline fruticosa ‘Purple’ and Aronia melanocarpa ‘Viking’ grown in either a temporary immersion system bioreactor or on conventional solid medium

Authors: John L Griffis *, Izzet Gün, Emrah Zeybekoglu, Mustafa Ercan Özzambak

There are numerous temporary immersion system (TIS) bioreactors available on the market. Various plants can benefit from the effects of the liquid medium immersion system on development and multiplication in vitro compared to their development and multiplication on a solid medium system with similar nutrients. However, not all bioreactors have the same design or working principles and not all plants respond positively to the TIS. This study aimed to evaluate and compare growth and multiplication effects of the Plantform Bioreactor culture system (TIS) with a conventional solid nutrient medium system using explants of Cordyline fruticosa 'Purple' and Aronia melanocarpa 'Viking' in vitro. The number of shoots per explant increased significantly in the Plantform Bioreactor system compared to the numbers produced per explant growing on solid medium for both cultivars. In Plantform Bioreactor culture, the multiplication rate was found to b e 8.9 shoots/explant for Cordyline fruticose 'Purple' and 11.9 shoots/explant for Aronia melanocarpa 'Viking'per transfe r. On solid medium, these values were identified as 2.2 shoots/explant for this cordyline and 6.6 shoots/explant for this aronia per transfer. However, Cordyline fruticosa 'Purple' also demonstrated both hyperhydricity symptoms and necrotic and curled leaves in the TIS that reduced plant quality. It appears that a direct comparison of in vitro nutrients between the systems does not provide an optimum growing condition for Cordyline fruticosa 'Purple' explants in the TIS, further system adjustments are necessary. For the aronia this was not the case: there were no abnormalities in plant growth and development of explants in the TIS when compared directly to explants grown on the solid medium system. The Plantform Bioreactor system (TIS) can be recommended for in vitro multiplication of Aronia melanocarpa 'Viking'.