S04 - Session P1 - New Tools I - Light effect on seed germination and growth of Curculigo latifolia in in vitro condition

Authors: Wirdhatul Muslihatin *, Amalia Salsabila, Nurul Jadid, Yosephine Sri Wulan Manohara, Stefaan Werbrouck

Curculigo latifolia (Lemba) is a member of the Hypoxidaceae family. It is a stemless perennial herb widely distributed in forests in Malaysia, Indonesia and Singapore. It produces fruits contains unique sweet proteins, namely curculin and neoculin, show promise as natural sweeteners. But, the seeds have a thick seed coat and are very difficult to germinate. Therefore, propagation in vitro is a good alternative. The purpose of this study was to determine the effect of light on germination in vitro. After external disinfection, the seed was grown on hormone-free MS medium containing 3% sucrose, 6% agar, and illuminated with red, blue, white LED, fluorescent light at a 16 h/8 h light-dark photoperiod and dark condition. The cultures were incubated at a temperature of 22 +- 2°C for 8 weeks. The result of this study showed that seeds of C. latifolia were photoblastic. They were germinated on light condition. Blue and white LED light produced higher plantlets and more number of leaves compared to other light treatments. For conclusion, light had an effect on germination and growth of C. latifolia Plantlets.