S01 - Session P5 - Screening of Capsicum annuum L. genotypes for resistance against pepper yellow leaf curl Thailand virus (PepYLCThV) by viruliferous whitefly inoculation

Authors: Nattika Kumsee *, Patcharaporn Suwor, Montinee Teerarak, Wen Shi Tsai, Suchila Techawongstien, Tanyarat Tainta, Sanjeet Kumar , Nakarin Jeeartid , Orawan Chatchawankanphanich, Somsak Kramchote

Chili pepper ( Capsicum annuum L.) is an economically imprtant crop worldwide. Pepper yellow leaf curl Thailand virus (PepYLCTHV), the causal agent of yellow mosaic disease of chili pepper belonging to the begomovirus group, has been reported causing crop losses under favorable epidemic conditions. Thirty-one chili genotypes were screened for resistance to PepYLCThV disease through inoculation using 10-15 viruliferous whiteflies per plant with a purified strain of the virus under 50 mesh insect-proof greenhouse conditions. Disease severity ranged from 0 to 4 for highly resistant to highly susceptible, respectively, were evaluated at 7 to 28 days after inoculation (DAI). At 28 DAI, two chili genotypes, PSP11-4 and 9853-123, showed the fewest symptoms at disease severity ratings of 3.64 and 3.83, respectively. In addition, both genotypes showed segregation of resistance and susceptibility (R:S): 9:21 for PSP11-4 and 7:23 for 9853-123. Viral genomic DNA was detected in all inoculated plants with scores 1-4, while in those with disease score of 0 viris was detected in some, but not in others. Therefore, the plants with a score of 0 may be of value for use in the chili breeding program.